Comparative adsorption of amylase, protease and lipase on ZnFe2O4: kinetics, isothermal and thermodynamics studies
نویسندگان
چکیده
The role of enzyme engineering in biotechnology, biological and pharmaceutical process cannot be over emphasized. This study compared the adsorption of digestives enzymes; amylase, protease and lipase on to Zn-ferrite (ZnFe2O4). The metal ferrite was synthesized via a sol-gel technique and characterized with scanning electron microscopy (SEM), X-ray diffraction (XRD), Electron paramagnetic resonance (EPR) and Fourier transform infrared spectroscopy (FTIR). The adsorption was studied in a batch process and the data were subjected to kinetics and isotherm models. Characterization shows that the particle has a nanoporous structure, with pore sizes of about 5.4 nm and good magnetic properties. The FTIR data showed the presence of M-O bond, which is a characteristic of metal ferrites. The adsorption of the amylase, lipase and protease on ZnFe2O4 follow first-order kinetic model with rate constants increasing with concentration. The maximum adsorption capacities as revealed by the generalized adsorption isotherms are 7.20, 42.90 and 22.24 mg g-1 for amylase, lipase and protease, respectively, with cooperative binding. The Dubinin-Radushkevich model gave the maximum adsorption energies, E of 3.74 kJ mol-1 for amylase, 2.01 kJ mol-1 for lipase and 1.51 kJ mol-1 for the protease adsorption, showing that the process is physisorption dominated. The isotherms fit the adsorption data in the order of Freundlinch > Generalized > Guggenheim-Anderson-de Boer > Tempkin isotherm > Dubinin-Radushkevich. Thermodynamic study revealed a spontaneous adsorption process with increased entropy. ZnFe2O4, therefore, is a very good adsorbent for the purification of enzymes and can be used as a supporter for enzymatic process that required immobilization of the enzymes.
منابع مشابه
Synthesis of MnFe2O4 nanoparticles for adsorption of digestive enzymes: Kinetics, isothermal and thermodynamics studies
The role of enzyme engineering in biotechnology, biological and pharmaceutical process cannot be over emphasized. This study compared the adsorption of amylase and protease on to manganese ferrite (MnFe2O4). The metal ferrite was synthesized via a sol – gel technique and characterized with scanning electron microscopy (SEM), X-ray diffraction (XRD), Electron paramagnet...
متن کاملKinetics and Isotherm Studies of the Immobilized Lipase on Chitosan Support
The kinetics and isotherm studies of the immobilized lipase and the mechanism of immobilization on chitosan beads and activated chitosan beads with glutaraldehyde were investigated. The effect of glutaraldehyde on porosity of chitosan was evaluated by FESEM analysis. It was observed that the porosity of the carrier which has activated by glutaraldehyde was substantially increased. The validity ...
متن کاملIsothermal and thermodynamic studies of the adsorption of safranine on MCM-41
The uses of MCM-41 as adsorbent have been studied as an alternative substitution of activated carbon for theremoval dyes. This adsorbent was prepared at laboratory by authors. Adsorption of safranine on MCM-41was studied to examine adsorption thermodynamics. In this study was investigated the effect of proccesparameters like pH, initial dye concentration, temperature and adsorbent mass. MCM-41,...
متن کاملAdsorption of Phenolic Compounds onto the Activated Carbon Synthesized from Pulp and Paper Mill Sludge: Equilibrium Isotherm, Kinetics, Thermodynamics and Mechanism Studies
A new activated carbon was produced from pulp and paper mill sludge as a low cost precursor via chemical activation by zinc chloride. The activated carbon was successfully used for the adsorption of phenol and two of its derivatives, i.e. 2-chlorophenol and 4-nitrophenol from aqueous solutions. The characterization of the prepared activated carbon demonstrated high textural properties for the s...
متن کاملKinetics, Thermodynamics and Equilibrium Studies on Adsorption of Reactive Red 198 from Textile Wastewater by Coral Limestone as a Natural Sorbent
Abstract Introduction: Dyes are among the most hazardous chemicals in industrial wastesdue to their toxicity for aquaculture, reduction of light permeability, and in turn development of disorders in photosynthesis process. The current study aimed to investigate the efficiency of coral in removing red reactive 198 from textile dye wastewater was investigated. Materials & Methods: In this e...
متن کامل